标题:Functional regulation of human trophoblast cells by CSE1L
作者:Zhou, Yu; Zhou, Qian; Li, Lei; Gu, Yongzhong; Sun, Xiaomei; Wang, Shan; Wang, Xietong
作者机构:[Zhou, Yu; Zhou, Qian; Li, Lei; Gu, Yongzhong; Sun, Xiaomei; Wang, Shan; Wang, Xietong] Shandong Univ, Shandong Prov Hosp, Dept Obstet & Gynaecol, 324 更多
通讯作者:Wang, XT
通讯作者地址:[Wang, XT]Shandong Univ, Shandong Prov Hosp, Dept Obstet & Gynaecol, 324 Jingwu St, Jinan, Shandong, Peoples R China.
来源:JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
DOI:10.1080/14767058.2019.1641482
关键词:Apoptosis; chromosomal segregation 1-like (CSE1L) gene; human placental; formation; human trophoblast cells; proliferation
摘要:Objectives: To investigate the effects of the human chromosomal segregation 1-like (CSE1L) gene on the biological behaviour of human placental cells. Study design: The CSE1L gene plays important roles in chromosome segregation during mitosis, cell proliferation and apoptosis. We used MTT, Cellomics, cell colony formation and flow cytometry assays in this study. Results: CSE1L mRNA was significantly increased in human placental cells (JAR) and decreased when CSE1L was knocked down. Lentiviral CSE1L knockdown reduced cell proliferation (p < .05) and colony formation (p = .00) and induced apoptosis (p < .000). CSE1L knockdown inhibited the G0/G1 phase (p = .00) and increased the G2/M phase (p = .00) of the cell cycle, but no significant change in the S phase was observed (p = .158). Conclusion: The CSE1L protein is expressed in human placental carcinoma cells, and its knockdown altered biological behaviours, implying that the CSE1L gene may affect human placental formation and even foetal development.
收录类别:SCIE
资源类型:期刊论文
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