作者机构：[Qin, J] State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;[ Xiao, Z] State Key Laboratory of Microbial Technolog 更多
通讯作者地址：[Xu, P]Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China.
关键词：2,3-butanediol production,optimization,fed-batch,Klebsiella pneumoniae
摘要：The production of 2,3-butanediol by Klebsiella pneumoniae from glucose supplemented with different salts was studied. A suitable medium composition was defined by response surface experiments. In a medium containing glucose and (NH4)2HPO4, the strain could convert 137.0g of glucose into 52.4g of 2,3-butanediol and 8.4g of acetoin in shaking flasks. The diol yield amounted to 90％ of its theoretical value and the productivity was 1-1.5g·L-1·h-1. In fed-batch fermentation, the yield and productivity of diol were further enhanced by maintaining the pH at 6.0. Up to 92.4g of 2,3-butanediol and 13.1g of acetoin per liter were obtained from 215.0g of glucose per liter. The diol yield reached 98% of its theoretical value and the productivity was up to 2.1g·L-1·h-1.