标题：Rapid determination of major bioactive isoflavonoid compounds during the extraction process of kudzu (Pueraria lobata) by near-infrared transmission spectroscopy
作者：Wang, Pei ;Zhang, Hui ;Yang, Hailong ;Nie, Lei ;Zang, Hengchang
作者机构：[Wang, Pei ;Yang, Hailong ;Nie, Lei ;Zang, Hengchang ] National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong Universit 更多
来源：Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
摘要：Near-infrared (NIR) spectroscopy has been developed into an indispensable tool for both academic research and industrial quality control in a wide field of applications. The feasibility of NIR spectroscopy to monitor the concentration of puerarin, daidzin, daidzein and total isoflavonoid (TIF) during the extraction process of kudzu (Pueraria lobata) was verified in this work. NIR spectra were collected in transmission mode and pretreated with smoothing and derivative. Partial least square regression (PLSR) was used to establish calibration models. Three different variable selection methods, including correlation coefficient method, interval partial least squares (iPLS), and successive projections algorithm (SPA) were performed and compared with models based on all of the variables. The results showed that the approach was very efficient and environmentally friendly for rapid determination of the four quality indices (QIs) in the kudzu extraction process. This method established may have the potential to be used as a process analytical technological (PAT) tool in the future.
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