标题：Visualized identification of the maximal surgical delay effect in a rat flap model
作者：Li, Xiucun; Chen, Miao; Jiang, Zhenmin; Liu, Yang; Lu, Laijin; Gong, Xu
作者机构：[Li, Xiucun] Shandong Univ, Hosp 2, Dept Hand Surg Foot & Ankle Surg, Jinan, Shandong, Peoples R China.; [Li, Xiucun; Chen, Miao; Jiang, Zhenmin; Li 更多
通讯作者地址：[Gong, X]Jilin Univ, Hosp 1, Dept Hand & Foot Surg, 71 Xinmin St, Changchun 130021, Jilin, Peoples R China.
来源：WOUND REPAIR AND REGENERATION
摘要：Currently, experimental evidence suggests that the surgical delay can increase flap survival area, but its effect may decrease if the optimal delay period is missed. The aim of this study is to establish a sensitive and objective modality based on the visualized and individualized infrared thermography for identifying the maximal surgical delay effect. A rectangular three-angiosome flap was designed on the unilateral dorsum of the rat. Ninety-six rats were randomly divided into six groups according to the various delay time. Both the relative temperature and the relative temperature ratio were measured by the infrared thermography. Arterial density, number of vessels >0.1 mm in diameter, microvessel density, VEGF concentration, and flap viability were measured. Receiving operating characteristic curve with the highest Youden-Index was used to detect and identify an optimal cutoff point of the relative temperature ratio in the maximal surgical delay effect. The criteria for identifying the flap maximum delay effect based on the infrared thermography included the surface of the postdelayed flaps presented white color (higher temperature) instead of the red and white pattern of the normal skin and the optimal cutoff point of the relative temperature ratio was 1.17 with a sensitivity of 84.6% and a specificity of 77.3%. Instead, the sensitivity and specificity of the conventional method based on the delay time were 38.5 and 90.9%, respectively. Infrared thermal imaging can accurately identify the maximum delay effect when combined with the relative temperature ratio.