标题:Synthesis, characterization of a novel phenyleneethynylene derivative and application in a fluorescence DNA sensor
作者:Chu, Chengchao; Wang, Xiu; Li, Shuai; Ge, Shenguang; Ge, Lei; Yu, Jinghua; Yan, Mei
作者机构:[Chu, Chengchao; Wang, Xiu; Li, Shuai; Ge, Shenguang; Ge, Lei; Yu, Jinghua; Yan, Mei] Univ Jinan, Shandong Univ, Sch Chem & Chem Engn, Key Lab Chem Se 更多
通讯作者:Yan, M
通讯作者地址:[Yan, M]Univ Jinan, Shandong Univ, Sch Chem & Chem Engn, Key Lab Chem Sensing & Anal, Jinan 250022, Peoples R China.
来源:ANALYTICAL METHODS
出版年:2012
卷:4
期:12
页码:4339-4345
DOI:10.1039/c2ay26086g
摘要:In this paper, a novel phenyleneethynylene derivative 4,4'-(2,5-dimethoxy-1,4-phenylene) bis(ethyne-2,1-diyl) dibenzoic acid (p-acid) was synthesized. Infrared spectroscopy (IR), nuclear magnetic resonance (NMR), photoluminescence (PL) spectroscopy and ultraviolet visible (UV-vis) spectroscopy were applied to characterize p-acid. The p-acid doped silica nanoparticle (p-acid@SiO2) was assembled in layer-by-layer (LBL) self-assembling processes using tetraethoxysilane (TEOS). Scanning electron microscope (SEM) and transmission electron microscope (TEM) were applied to characterize p-acid@SiO2. To research the practical applicability, a sample DNA sensor was constructed using p-acid@SiO2 label, in which the PL intensity response was proportion to the target-DNA (S-2) concentration in the range of 0.05-1000 fM, with a detection limit of 20 pM. Furthermore, the DNA sensor showed high specificity, excellent stability, and good reproducibility. The p-acid@SiO2-based DNA sensor can also provide potential application for detection of other pathogen DNA.
收录类别:EI;SCIE
WOS核心被引频次:3
资源类型:期刊论文
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