标题：Through-bond energy transfer-based ratiometric fluorescent probe for the imaging of HOCl in living cells
作者：Shen, Shi-Li ;Ning, Jun-Ya ;Zhang, Xiao-Fan ;Miao, Jun-Ying ;Zhao, Bao-Xiang
作者机构：[Shen, Shi-Li ;Zhang, Xiao-Fan ;Zhao, Bao-Xiang ] Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, J 更多
来源：Sensors and Actuators, B: Chemical
关键词：Bioimaging; Fluorescent probe; Hypochlorous acid; Ratiometric; Through-bond energy transfer
摘要：A ratiometric fluorescent probe (NR) for HOCl based on a new naphthalimide-rhodamine platform involving through-bond energy transfer (TBET) process is presented. Rhodamine B thiohydrazide and naphthalimide derivative were linked to form a structure of monothio-bishydrazide. Free NR only emitted the fluorescence of naphthalimide. The treatment of NR with HOCl converted the monothio-bishydrazide into 1,2,4-oxadiazole. As a result, naphthalimide donor and rhodamine acceptor were connected with electronically conjugated bonds, and the spiro ring of rhodamine was opened. Hence, a typical TBET process took place, resulting in the increase of rhodamine emission at 582 nm as well as the decrease of naphthalimide emission at 532 nm. I582/I532ratios showed desirable HOCl-dependent performance and responded linearly to minor changes of HOCl concentration (0–5 μM). The detection limit was determined to be 14.5 nM. NR exhibited high selectivity, rapid response and low cytotoxicity. Moreover, NR has been employed for the imaging of artificially loaded HOCl and endogenously produced HOCl with satisfying results. Therefore, NR may act as a practical molecular tool for monitoring HOCl fluctuations in biological systems. © 2017 Elsevier B.V.