标题:USP35 activated by miR let-7a inhibits cell proliferation and NF-kappa B activation through stabilization of ABIN-2
作者:Liu, Chunyan; Wang, Lina; Chen, Weiwen; Zhao, Shihu; Yin, Chunli; Lin, Yani; Jiang, Anli; Zhang, Pengju
作者机构:[Liu, Chunyan; Wang, Lina; Chen, Weiwen; Zhao, Shihu; Yin, Chunli; Lin, Yani; Jiang, Anli; Zhang, Pengju] Shandong Univ, Sch Med, Dept Biochem & Mol B 更多
通讯作者:Zhang, PJ
通讯作者地址:[Zhang, PJ]Shandong Univ, Sch Med, Dept Biochem & Mol Biol, Jinan 250012, Shandong, Peoples R China.
来源:ONCOTARGET
出版年:2015
卷:6
期:29
页码:27891-27906
DOI:10.18632/oncotarget.4451
关键词:ABIN-2; deubiquitylase; miR let-7a; NF-kappa B; USP35
摘要:Ubiquitin specific protease 35 (USP35) is a member of deubiquitylases (DUBs). It remains largely unknown about the biological role and the regulation mechanism of USP35. Here, we first identified miR let-7a as a positive regulator of USP35 expression and showed that USP35 expression positively correlates with miR let-7a expression in different cancer cell lines and tissues. Then, we showed that USP35 expression was decreased dramatically in the tumor tissues compared with the adjacent non-cancerous tissues. USP35 overexpression inhibited cell proliferation in vitro and inhibited xenograft tumor growth in vivo. Furthermore, we revealed that USP35 acts as a functional DUB and stabilizes TNFAIP3 interacting protein 2 (ABIN-2) by promoting its deubiquitination. Functionally, both ABIN-2 and USP35 could inhibit TNF alpha-induced NF kappa B activation and overexpression of ABIN-2 alleviated USP35-loss induced activation of NF-kappa B. Collectively, our data indicated that miR let-7a-regulated USP35 can inhibit NF-kappa B activation by deubiquitination and stabilization of ABIN-2 protein and eventually inhibit cell proliferation. Overall, our study provides a novel rationale of targeting miR let-7a-USP35-ABIN-2 pathway for the therapy of cancer patients.
收录类别:SCIE
WOS核心被引频次:6
资源类型:期刊论文
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