标题：The composition and chemical alteration of gums in the vessels of Phellodendron amurense
作者：Zheng, Peiming; Yagami, Sachie; Aoki, Dan; Yoshida, Masato; Sano, Yuzou; Matsushita, Yasuyuki; Fukushima, Kazuhiko
作者机构：[Aoki, Dan] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan.; [Sano, Yuzou] Hokkaido Univ, Res Fac Agr, Sapporo, Hokkaido 更多
通讯作者地址：[Aoki, D]Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan;[Sano, Y]Hokkaido Univ, Res Fac Agr, Sapporo, Hokkaido 0608589, Ja 更多
关键词：gum; heartwood; laser microdissection; Phellodendron amurense; Raman; microscopy; sapwood
摘要：An occluding substance (gum) was observed in the vessels of Phellodendron amurense Rupr. and analysed by spectroscopic and chemical methods. Following safranin-alcian blue staining, the gum in sapwood (sW) turned to blue and in heartwood (hW) to red. The gum was studied in situ by UV and Raman microscopies, time-of-flight secondary ion mass spectrometry (TOF-SIMS). The gum was isolated by laser microdissection (LMD) and it was alkali hydrolysed and the degradation products were analysed by GC-MS. The staining experiments, and the UV and Raman microscopies indicated that the major component of the sW gum is constituted of polysaccharides, while in the hW gum the aromatic character is dominating. TOF-SIMS measurements were interpreted as showing the aromatic substances in the hW gum did not contain lignin. The GC-MS analysis revealed the presence of vanillic acid in the degradation products of hW gum.