标题：Efficacy of dual-functional liposomes containing paclitaxel for treatment of lung cancer
作者：Wang, Rong-Hua;Cao, Hong-Mei;Tian, Zhi-Ju;Jin, Bo;Wang, Qing;Ma, Hong;Wu, Jing
作者机构：[Wang, R.-H] Department of Cardiothoracic Surgery, People's Hospital of Zhangqiu, Huiquan Road 1920, Zhangqiu, Shandong, 250200, China;[ Cao, H.-M] De 更多
通讯作者地址：[Wang, RH]Peoples Hosp Zhangqiu, Dept Cardiothorac Surg, Huiquan Rd 1920, Zhangqiu 250200, Shandong, Peoples R China.
关键词：dual-ligand;cell-penetrating peptide;transferrin receptor;liposome
摘要：This study was mainly focused on the development of a dual-ligand liposomal delivery system for targeting the delivery of paclitaxel (PTX) to lung cancer. The specific ligand peptide HAIYPRH (T7) and the cationic cell-penetrating peptide TAT were connected with phospholipid via a polyethylene glycol (PEG) spacer to prepare the dual-ligand liposomes (T7/TAT-LP-PTX). Physicochemical characterizations of T7/TAT-LP-PTX, such as particle size, zeta potential, morphology, encapsulation efficiency, and in vitro PTX release, were also evaluated. In the cellular uptake study, the T7/TAT-LP endocytosed by the A549 cells was 2.26-, 3.48- and 8.56-fold higher than TAT-LP, T7-LP and LP, respectively. The IC50 values of TAT-LP-PTX, T7-LP-PTX and LP-PTX were much higher than those of T7/TAT-LP-PTX, respectively. The homing specificity of T7/TAT-LP was evaluated on the tumor spheroids, which revealed that T7/TAT-LP was more efficaciously internalized in tumor cells than TAT-LP, T7-LP and LP, respectively. Compared to LP, TAT-LP and T7-LP, T7/TAT-LP showed the strongest cell uptake property, and the highest accumulation ability in tumor spheroids in vitro. In the in vivo study, the T7/TAT-LP-PTX exhibited the best inhibitory effect of tumor growth for A549-bearing mice. Collectively, these results suggested that T7/TAT-LP-PTX is a promising drug delivery system for the treatment of lung cancer.