标题:Efficacy of dual-functional liposomes containing paclitaxel for treatment of lung cancer
作者:Wang, Rong-Hua;Cao, Hong-Mei;Tian, Zhi-Ju;Jin, Bo;Wang, Qing;Ma, Hong;Wu, Jing
作者机构:[Wang, R.-H] Department of Cardiothoracic Surgery, People's Hospital of Zhangqiu, Huiquan Road 1920, Zhangqiu, Shandong, 250200, China;[ Cao, H.-M] De 更多
通讯作者:Wang, RH
通讯作者地址:[Wang, RH]Peoples Hosp Zhangqiu, Dept Cardiothorac Surg, Huiquan Rd 1920, Zhangqiu 250200, Shandong, Peoples R China.
来源:Oncology reports
出版年:2015
卷:33
期:2
页码:783-791
DOI:10.3892/or.2014.3644
关键词:dual-ligand;cell-penetrating peptide;transferrin receptor;liposome
摘要:This study was mainly focused on the development of a dual-ligand liposomal delivery system for targeting the delivery of paclitaxel (PTX) to lung cancer. The specific ligand peptide HAIYPRH (T7) and the cationic cell-penetrating peptide TAT were connected with phospholipid via a polyethylene glycol (PEG) spacer to prepare the dual-ligand liposomes (T7/TAT-LP-PTX). Physicochemical characterizations of T7/TAT-LP-PTX, such as particle size, zeta potential, morphology, encapsulation efficiency, and in vitro PTX release, were also evaluated. In the cellular uptake study, the T7/TAT-LP endocytosed by the A549 cells was 2.26-, 3.48- and 8.56-fold higher than TAT-LP, T7-LP and LP, respectively. The IC50 values of TAT-LP-PTX, T7-LP-PTX and LP-PTX were much higher than those of T7/TAT-LP-PTX, respectively. The homing specificity of T7/TAT-LP was evaluated on the tumor spheroids, which revealed that T7/TAT-LP was more efficaciously internalized in tumor cells than TAT-LP, T7-LP and LP, respectively. Compared to LP, TAT-LP and T7-LP, T7/TAT-LP showed the strongest cell uptake property, and the highest accumulation ability in tumor spheroids in vitro. In the in vivo study, the T7/TAT-LP-PTX exhibited the best inhibitory effect of tumor growth for A549-bearing mice. Collectively, these results suggested that T7/TAT-LP-PTX is a promising drug delivery system for the treatment of lung cancer.
收录类别:SCOPUS;SCIE
WOS核心被引频次:8
Scopus被引频次:10
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84919458795&doi=10.3892%2for.2014.3644&partnerID=40&md5=e3c00fcd6d3ddd4fa161f51e439945b8
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