标题：Ampelopsin reduces the migration and invasion of ovarian cancer cells via inhibition of epithelial-to-mesenchymal transition
作者：Liu, Tianfeng;Liu, Peishu;Ding, Feng;Yu, Nina;Li, Shihong;Wang, Surong;Zhang, Xiaofei;Sun, Xiangxiu;Chen, Ying;Wang, Feng;Zhao
作者机构：Shandong Univ, Dept Gynecol, Qilu Hosp, Jinan 250012, Shandong, Peoples R China;Shandong Univ, Dept Gynecol, Qilu Hosp, Jinan 25
通讯作者地址：[Liu, PS]Shandong Univ, Dept Gynecol, Qilu Hosp, 107 West Culture Rd, Jinan 250012, Shandong, Peoples R China.
关键词：ampelopsin;NF-kappa B;Snail;epithelial-to-mesenchymal transition
摘要：Ampelopsin has displayed anticancer activity in several types of cancers. However, no evidence has been reported for the direct effect of ampelopsin on ovarian cancer cell migration and invasion, and the underling mechanisms have not yet been clearly established. The aim of the present study was to investigate the influence of ampelopsin on the migration and invasion of ovarian cancer. Proliferation and viability of the ovarian cancer cells were detected by MTT assay. Migration and invasion of the cells were detected, respectively, by scratch wound healing assay and Transwell assay. The expression levels of epithelial-to-mesenchymal transition (EMT) markers were detected at the protein level after stimulation with ampelopsin. Then, the expression levels of NF-kappa B and p-I kappa B alpha were detected with western blot analysis. Meanwhile, an inhibitor of NF-kappa B was used to investigate the effect of ampelopsin. Finally, the expression of Snail was also detected. Proliferation, migration and invasion of the A2780 cells were all inhibited following the application of ampelopsin. Ampelopsin upregulated E-cadherin and down-regulated N-cadherin and vimentin in a concentration- and time-dependent manner. Ampelopsin also exerted its ability to suppress the nuclear translocation of the NF-kappa B pathway. Administration of the inhibitor BAY11-7082 confirmed the roles of NF-kappa B in the expression of EMT markers and its transcription factor. These results demonstrated that ampelopsin inhibited EMT and reduced the invasion of ovarian cancer cells via the NF-kappa B/Snail pathway.