标题：Status epilepticus increases mature granule cells in the molecular layer of the dentate gyrus in rats
作者：Liang, Zhaoliang; Gao, Fei; Wang, Fajun; Wang, Xiaochen; Song, Xinyu; Liu, Kejing; Zhan, Ren-Zhi
作者机构：[Liang Zhaoliang] Institute of Physiology, Shandong University School of Medicine, Jinan, Shandong 250012, China.;[Gao Fei] Institute of Physiology, S 更多
通讯作者地址：[Zhan, RZ]Shandong Univ, Sch Med, Inst Physiol, Jinan 250012, Shandong, Peoples R China.
关键词：neural regeneration;basic research;status epilepticus;hippocampus;dentate gyrus;granule cells;molecular layer;prospero homeobox protein 1;neuron-specific nuclear protein;doublecortin;grants-supported paper;photographs-containing paper;neuroregen
摘要：Enhanced neurogenesis in the dentate gyrus of the hippocampus following seizure activity, especially status epilepticus, is associated with ectopic residence and aberrant integration of newborn granule cells. Hilar ectopic granule cells may be detrimental to the stability of dentate circuitry by means of their electrophysiological properties and synaptic connectivity. We hypothesized that status epilepticus also increases ectopic granule cells in the molecular layer. Status epilepticus was induced in male Sprague-Dawley rats by intraperitoneal injection of pilocarpine. Immunostaining showed that many doublecortin-positive cells were present in the molecular layer and the hilus 7 days after the induction of status epilepticus. At least 10 weeks after status epilepticus, the estimated number of cells positive for both prospero homeobox protein 1 and neuron-specific nuclear protein in the hilus was significantly increased. A similar trend was also found in the molecular layer. These findings indicate that status epilepticus can increase the numbers of mature and ectopic newborn granule cells in the molecular layer.