标题：Study on isolation of cellulose-decomposing microorganisms and its cellulase-producing conditions
作者：Sun, Qing ;Yi, Weiming ;Gu, Shiyan ;Zhang, Chunmei ;Ge, Jingfeng ;Liu, Qingyu
作者机构：[Sun, Qing ;Gu, Shiyan ;Zhang, Chunmei ;Ge, Jingfeng ;Liu, Qingyu ] Engineering College, Shenyang Agricultural University, Shenyang 110161, China;[Yi, 更多
来源：Nongye Jixie Xuebao/Transactions of the Chinese Society of Agricultural Machinery
摘要：According to the theory of microbial ecology, with sweet sorghum straw as the raw material, cellulose-decomposing fungi, which had high enzyme activity, were isolated from their natural habitat and screened. Meanwhile, the production conditions of cellulose enzyme were optimized based on the factors of temperature, initial pH value, and inoculation quantity, and the influence of these factors on enzyme activity was discussed. Taking Z15 as a test strain, optimization of production capacity of crude enzyme test was carried out, according to three factors, five-level orthogonal rotational regression test design, to investigate the effects on fermentation temperature, initial pH value and inoculation quantity. The yield of reducing sugar was taken as the indicators, and the impact on the target size about the three factors was analyzed. By calculating the analysis, the following results of the impact on the target was obtained: temperature was the first one, inoculation quantity was the last one and initial pH value was the middle. The optimum conditions for enzymatic hydrolysis were obtained: optimum temperature was 26.64°C, optimum initial pH value was 4.96, and optimum inoculation quantity was 5%, at this time reducing sugar content was 54.19 mg/g.