标题：Lighting up bioluminescence with coelenterazine: strategies and applications
作者：Jiang, Tianyu; Du, Lupei; Li, Minyong
作者机构：[Jiang, Tianyu; Du, Lupei; Li, Minyong] Shandong Univ, Sch Pharmaceut Sci, Key Lab Chem Biol MOE, Dept Med Chem, Jinan 250012, Shandong, Peoples R Chi 更多
通讯作者地址：[Li, MY]Shandong Univ, Sch Pharmaceut Sci, Key Lab Chem Biol MOE, Dept Med Chem, Jinan 250012, Shandong, Peoples R China.
来源：PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
摘要：Bioluminescence-based techniques, such as bioluminescence imaging, BRET and dual-luciferase reporter assay systems, have been widely used to examine a myriad of biological processes. Coelenterazine (CTZ), a luciferin or light-producing compound found in bioluminescent organisms, has sparked great curiosity and interest in searching for analogues with improved photochemical properties. This review summarizes the current development of coelenterazine analogues, their bioluminescence properties, and the rational design of caged coelenterazine towards biotargets, as well as their applications in bioassays. It should be emphasized that the design of caged luciferins can provide valuable insight into detailed molecular processes in organisms and will be a trend in the development of bioluminescent molecules.