标题:MicroRNA-186-5p Inhibits Proliferation And Metastasis Of Esophageal Cancer By Mediating HOXA9
作者:Xu, Changqin; Li, Bin; Zhao, Shulei; Jin, Bingjie; Jia, Ruzhen; Ge, Jian; Xu, Hongwei
作者机构:[Xu, Changqin; Li, Bin; Zhao, Shulei; Jin, Bingjie; Jia, Ruzhen; Ge, Jian; Xu, Hongwei] Shandong Univ, Dept Gastroenterol, Shandong Prov Hosp, 324 Jin 更多
通讯作者:Xu, HW
通讯作者地址:[Xu, HW]Shandong Univ, Dept Gastroenterol, Shandong Prov Hosp, 324 Jingwu Rd, Jinan 250021, Shandong, Peoples R China.
来源:ONCOTARGETS AND THERAPY
出版年:2019
卷:12
页码:8905-8914
DOI:10.2147/OTT.S227920
关键词:microRNA-186-5p; HOXA9; esophageal cancer; proliferation; metastasis
摘要:Objective: MicroRNA (miRNA) is an endogenous, non-coding small RNA that plays a key role in regulating organism biology and pathology. The aim of this study was to investigate the expression characteristics of microRNA-186-5p in esophageal cancer (ECa) and its correlation with clinical progression and prognosis, and to further explore its underlying mechanisms.; Methods: Real-time quantitative PCR (qRT-PCR) was used to detect microRNA-186-5p level in 45 pairs of ECa tissue samples and adjacent ones, and to analyze the expression of microRNA-186-5p and clinical progression of ECa and prognosis. The relationship between microRNA-186-5p level in ECa cell lines was further verified by qRT-PCR. Finally, the potential mechanism was explored using luciferase reporter gene assay and cell recovery experiment.; Results: QRT-PCR results revealed that the expression of microRNA-186-5p in ECa tissues was remarkably lower than that in adjacent tissues, and the difference was statistically significant. Compared with patients with high expression of microRNA-186-5p, patients with low expression of microRNA-186-5p had higher incidence of pathological stage and lower overall survival rate. Besides, compared with the miR-NC group, the microRNA-186-5p mimics group had a significant decrease in proliferation and metastasis ability of ECa cells. Subsequent qRT-PCR validation in ECa cell lines and tissues indicated a significant increase in HOXA9 expression and a negative correlation with microRNA-186-5p.; Conclusion: The expression of microRNA-186-5p was remarkably decreased in ECa, which was remarkably correlated with pathological stage, distant metastasis and poor prognosis of ECa. The results suggested that microRNA-186-5p may inhibit cell proliferation of ECa by regulating HOXA9.
收录类别:SCOPUS;SCIE
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074320707&doi=10.2147%2fOTT.S227920&partnerID=40&md5=606ea240f2f8b9d564e9834bf36357fd
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