标题：Elevation of 4-hydroxynonenal and malondialdehyde modified protein levels in cerebral cortex with cognitive dysfunction in rats exposed to 1-bromopropane
作者：Zhong, Zhixia; Zeng, Tao; Xie, Keqin; Zhang, Cuili; Chen, Jingjing; Bi, Ye; Zhao, Xiulan
作者机构：[Zhong, Zhixia; Zeng, Tao; Xie, Keqin; Zhang, Cuili; Chen, Jingjing; Bi, Ye; Zhao, Xiulan] Shandong Univ, Sch Publ Hlth, Inst Toxicol, Jinan 250012, S 更多
通讯作者地址：[Zhao, XL]Shandong Univ, Sch Publ Hlth, Inst Toxicol, Jinan 250012, Shandong, Peoples R China.
关键词：1-Bromopropane; Central nervous system; Oxidative stress;; 4-Hydroxynonenal; Malondialdehyde
摘要：1-Bromopropane (1-BP), an alternative to ozone-depleting solvents (ODS), exhibits central nervous system (CNS) toxicity in animals and humans. This study was designed to relate CNS damage by Morris water maze (MWM) test and oxidative stress to 1-BP exposure in the rat. Male Wistar rats were randomly divided into 4 groups (n = 10), and treated with 0, 200, 400 and 800 mg/kg bw 1-BP for consecutive 12 days, respectively. From day 8 to day 12 of the experiment, MWM test was employed to assess the cognitive function of rats. The cerebral cortex of rats was obtained immediately following the 24 h after MWM test conclusion. Glutathione (GSH), oxidized glutathione (GSSG) and total thiol (total-SH) content, GSH reductase (GR) and GSH peroxidase (GSH-Px) activities, malondialdehyde (MDA) level, as well as 4-hydroxynonenal (4-HNE) and MDA modified proteins in homogenates of cerebral cortex were measured. The obtained results showed that 1-BP led to cognitive dysfunction of rats, which was evidenced by delayed escape latency time and swimming distances in MWM performance. GSH and total-SH content, GSH/GSSG ratio, GR activity significantly decreased in cerebral cortex of rats, coupling with the increase of MDA level. 4-HNE and MDA modified protein levels obviously elevated after 1-BP exposure. GSH-Px activities in cerebral cortex of rats also increased. These data suggested that 1-BP resulted in enhanced lipid peroxidation of brain, which might play an important role in CNS damage induced by 1-BP. (C) 2013 Elsevier Ireland Ltd. All rights reserved.