标题：Cervical Cancer Gene Therapy by Gene Loaded PEG-PLA Nanomedicine
作者：Liu, Bo; Han, Shu-Mei; Tang, Xiao-Yong; Han, Li; Li, Chang-Zhong
作者机构：[Liu, Bo; Han, Shu-Mei; Tang, Xiao-Yong; Han, Li] Shandong Tumor Hosp & Inst, Dept Internal Med Oncol, Jinan, Peoples R China.; [Li, Chang-Zhong] Sh 更多
通讯作者地址：[Li, CZ]Shandong Univ, Shandong Prov Hosp, Dept Gynecol, Jinan 250100, Peoples R China.
来源：ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
关键词：Gene therapy; cervical cancer; folate coating; targeted nanomedicine
摘要：Background and Aims: Advances in the treatment of cervical cancer over the last decade have predominantly involved the development of genes directed at molecular targets. Gene therapy is recognized to be a novel method for the treatment of cervical cancer. Genes can be administered into target cells via nanocarriers. This study aimed to develop systemically administrable nano-vectors. Floate (Fa) containing gene loaded nanoparticles (NPs) could target HeLa human cervical cancer cells through combination with receptors on the cells to increase the nuclear uptake of genetic materials. Methods: Fa was linked onto Poly (ethylene glycol)-b-poly (D,L-lactide) (PEG-PLA) to form Fa-PEG-PLA, and the resulting material was used to load plasmids of enhanced green fluorescence protein (pEGFP) to obtain gene loaded nanoparticles (Fa-NPs/DNA). Physical-chemical characteristics, in vitro release and cytotoxicity of Fa-NPs/DNA were evaluated. The in vitro transfection efficiency of Fa-NPs/DNA was evaluated in HeLa cells and human umbilical vein endothelial cells (HUVEC). PEG-PLA without Fa was used to load pEGFP from NPs/DNA as a control. Results: Fa-NPs/DNA has a particle size of 183 nm and a gene loading quantity of 92%. After 72h of transfection, Fa-NPs/DNA displayed over 20% higher transfection efficiency than NPs/DNA and 40% higher than naked DNA in HeLa cells. However, in HUVECs, no significant difference appeared between Fa-NPs/DNA and NPs/DNA. Conclusions: Fa-PEG-PLA NPs could function as excellent materials for gene loading. This nano-approach could be used as tumor cell targeted medicine for the treatment of cervical cancer.