标题:ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHE Lexpression
作者:Xuehuan Dai[1];Zhenhua Liu[1];Meng Qiao[2];Juan Li[1];Shuo Li[1];Fengning Xiang[1]
作者机构:[Dai, X] The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Jinan, 250100, China;[ L 更多
通讯作者:Xiang, F(xfn0990@sdu.edu.cn)
通讯作者地址:[Xiang, FN]Shandong Univ, Key Lab Plant Cell Engn & Germplasm Innovat, Sch Life Sci, Jinan 250100, Shandong, Peoples R China.
来源:植物学报:英文版
出版年:2017
卷:59
期:10
页码:747-758
DOI:10.1111/jipb.12567
关键词:ARR12;PROMOTES;WUSCHE;Lexpression;
摘要:Auxin and cytokinin direct cell proliferation anddifferentiation during the in vitro culture of plant cells, but themolecular basis of these processes, especially de novo shootregeneration, has not been fully elucidated. Here, we describethe regulatory control of shoot regeneration in Arabidopsisthaliana (L.) Heynh, based on the interaction of ARABIDOPSISRESPONSE REGULATOR12 (ARR12) and WUSCHEL (WUS). Themajor site of ARR12 expression coincided with the locationwhere the shoot apical meristem (SAM) initiated. The arr12mutants showed severely impaired shoot regeneration andreduced responsiveness to cytokinin; consistent with this, theoverexpression of ARR12 enhanced shoot regeneration.Certain shoot meristem specification genes, notably WUSCHEL(WUS) and CLAVATA3, were significantly downregulated in thearr12 explants. Chrornatin immunoprecipitation (CHIP) andtransient activation assays demonstrated that ARR12 binds tothe promoter of WUS. These observations indicate that duringshoot regeneration, in vitro, ARR12 functions as a molecularlink between cytokinin signaling and the expression of shootmeristem specification genes.
收录类别:CSCD;SCOPUS;SCIE
WOS核心被引频次:4
Scopus被引频次:4
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030681840&doi=10.1111%2fjipb.12567&partnerID=40&md5=7de6c5ff2ef342ea4e7688a9856b2c62
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