标题:Analysis of heparin oligosaccharides by capillary electrophoresis-negative-ion electrospray ionization mass spectrometry
作者:Lin, Lei; Liu, Xinyue; Zhang, Fuming; Chi, Lianli; Amster, I. Jonathan; Leach, Franklyn E., III; Xia, Qiangwei; Linhardt, Robert J.
作者机构:[Lin, Lei; Liu, Xinyue; Zhang, Fuming; Linhardt, Robert J.] Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Dept Chem & Chem Bio 更多
通讯作者:Linhardt, Robert J
通讯作者地址:[Linhardt, RJ]Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Dept Chem & Chem Biol, 110 Eighth St, Troy, NY 12180 USA;[Linhardt 更多
来源:ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版年:2017
卷:409
期:2
页码:411-420
DOI:10.1007/s00216-016-9662-1
关键词:Hyphenated techniques; CE-MS; Heparin; Low molecular weight heparin;; Oligosaccharides; Glycosaminoglycan
摘要:Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization. Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source. Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid. The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin. The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:7
Scopus被引频次:9
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975217691&doi=10.1007%2fs00216-016-9662-1&partnerID=40&md5=37f8cca7f996ffe4f445be5226864119
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