标题：Discovery and characterization of a stable lipase with preference toward long-chain fatty acids
作者：Chen, Meiling; Gao, Xiuzhen; Yang, Wu; Sun, Caili; Yang, Junhuan; Zhang, Huaiyuan; Song, Yuanda
作者机构：[Chen, Meiling; Yang, Wu; Sun, Caili; Yang, Junhuan; Zhang, Huaiyuan; Song, Yuanda] Shandong Univ Technol, Sch Agr Engn & Food Sci, Colin Ratledge Ctr 更多
通讯作者：Song, Yuanda;Song, YD;Gao, XZ
通讯作者地址：[Song, YD]Shandong Univ Technol, Sch Agr Engn & Food Sci, Colin Ratledge Ctr Microbial Lipids, Zibo 255000, Peoples R China;[Gao, XZ]Shandong Univ Tec 更多
关键词：CALA; Enrichment; Lipase; Mucor circinelloides; Phylogeny analysis;; Unsaturated fatty acid
摘要：Objectives To identify novel lipases with stability and long-chain fatty acids preference by phylogenetic evolution analysis methods from database.; Results Thermo-stable Candida antarctica Lipase-A (CALA) was set as a template for gene mining by PSI-BLAST. Based on phylogenetic analysis, three candidate lipases exhibiting 97%, 55%, and 35% identities with CALA, respectively, were selected for overexpression and characterization. Lipase, PhLip from Pseudozyma hubeiensis SY62 showed highest activity towards long-chain fatty acids, and showed maximum activity at pH 9.0 and 60 degrees C, and stability between 40 and 50 degrees C for 4 h and at pH 7-10 for 12 h. Enzymatic hydrolysis of Mucor circinelloides WJ11 oils by PhLip was about twofold higher than that by CALA, with respect to hydrolysis of long-chain fatty acids. Besides, fatty acids with 18 carbons, including oleic acid, linoleic acid, and linolenic acid, were preferred as substrates.; Conclusion The current investigation discovered a stable lipase PhLip with long-chain fatty acids preference. PhLip may be a potential candidate for producing polyunsaturated fatty acids from natural oils.