标题:Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. strigosa
作者:Qin, Lumin;Liang, Yu;Yang, Daozheng;Xia, Guangmin;Liu, Shuwei
作者机构:Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ, Jinan 250100, Peoples R China;Shandon
通讯作者:Liu, SW
通讯作者地址:[Liu, SW]Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ, Jinan 250100, Peoples R China.
来源:Journal of Applied Genetics
出版年:2015
卷:56
期:1
页码:27-35
DOI:10.1007/s13353-014-0229-6
关键词:LMW-GS;Unequal crossing over;Pseudoroegneria;St genome;Illegitimate recombination
摘要:We report the characterisation of nine novel low molecular weight glutenin subunit (LMW-GS) genes from two Pseudoroegneria species, Pd. spicata and Pd. strigosa. We found that all nine LMW-GS genes possess the same primary structure shared by other published LMW-GS. Five genes encode LMW-i type subunits, three encode LMW-m type subunits and one encodes a peptide similar to B-3 hordeins of Hordeum chilense. No LMW-s type subunit genes were found in Pseudoroegneria. One subunit, PSt24-LMW-2, contains six conserved cysteine residues, and the other eight subunits all contain eight cysteine residues. We show that one cysteine residue is located in the signal peptide of PSt24-LMW-1, suggesting a mature peptide containing only seven cysteine residues. Phylogenetic analysis indicates that the LMW-GS genes from the St genome cluster together and suggests a distant relationship with LMW-GS of the A and B genomes of wheat. Slippage/unequal crossing over and illegitimate recombination are effective mechanisms for enriching variations of seed storage proteins.
收录类别:SCIE
资源类型:期刊论文
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