标题：Cordycepin and pentostatin biosynthesis gene identified through transcriptome and proteomics analysis of Cordyceps kyushuensis Kob
作者：Zhao, Xuan; Zhang, Guoying; Li, Caiyi; Ling, Jianya
作者机构：[Zhao, Xuan; Li, Caiyi; Ling, Jianya] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China.; [Zhang, Guoying] Sha 更多
通讯作者：Ling, Jianya;Ling, JY
通讯作者地址：[Ling, JY]Shandong Univ, State Key Lab Microbial Technol, Sch Life Sci, Jinan, Shandong, Peoples R China.
关键词：Transcriptome; Proteomics; Cordycepin; Pentostatin; HPLC analysis
摘要：Cordyceps kyushuensis is the only species of cordyceps growing on the larvae of Cianis bilineata Walker, and has been demonstrated that there are lots of pharmacological components including cordycepin. Cordycepin shows lots of pharmacological action but it could be converted to 3'-deoxyinosine by adenosine deaminase in vivo, which weakens the efficiency of cordycepin. That pentostatin, which has been reported to inhibit adenosine deaminase, combining cordycepin could enhance the efficiency of cordycepin in vivo. During transcriptome and proteomics analysis of Cordyceps kyushuensis, a single gene cluster including four genes we named ck1-ck4 which can synthesis both cordycepin and pentostatin has been identified using BLAST. Meanwhile, KEGG, KOG, GO analysis and differentially expressed genes were analyzed in transcriptome and proteomics. This study first sequenced transcriptome and proteomics of C. kyushuensis, and demonstrated that there is a single gene cluster related to biosynthesis of cordycepin and pentostatin, which can be employed to improve the yield of cordycepin and find more functional proteins.