标题：Cyclodextrin glucanotransferase: from gene to applications
作者：Qi, QS; Zimmermann, W
作者机构：[Qi, QS]Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China.;[ Zimmermann, W] Tech Univ Chemnitz, Dept Bioproc Technol, D-09107 Chemnitz, G 更多
通讯作者地址：[Qi, QS]Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China.
来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
摘要：Cyclodextrin glucanotransferase (CGTase) is an important industrial enzyme which is used to produce cyclodextrins. CGTase genes from more than 30 bacteria have been isolated and several of the enzymes have been identified and biochemically characterized. For a better understanding of the reaction mechanism and function of CGTase, the enzyme has been analyzed at gene level and protein level with regard to its structure and the similarity of different CGTase subgroups. The biological role of the enzyme is proposed based on the genetic and enzymatic analyses. Methods to enhance the production of active CGTase by bacteria are compared. The enzyme can be applied in biotechnology for the production of cyclodextrins and oligosaccharides with novel properties.