标题:Aptamer-functionalized nanoporous gold film for high-performance direct electrochemical detection of bisphenol A in human serum
作者:Zhu, Ye; Zhou, Chuqing; Yan, Xupeng; Yan, Yan; Wang, Qiang
作者机构:[Zhu, Ye; Zhou, Chuqing; Yan, Xupeng; Yan, Yan] Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.; [Wang, Qiang] Tianjin Univ Tech 更多
通讯作者:Zhu, Ye
通讯作者地址:[Zhu, Y]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
来源:ANALYTICA CHIMICA ACTA
出版年:2015
卷:883
页码:81-89
DOI:10.1016/j.aca.2015.05.002
关键词:Nanoporous gold; Aptamer sensor; Bisphenol A; Direct electrochemical; detection; Electrocatalysis
摘要:In the present work, a highly sensitive and selective biosensor based on aptamer-functionalized nanoporous gold film (NPGF) was successfully developed for direct electrochemical detection of bisphenol A (BPA). NPGF was prepared by dealloying Ag from Au/Ag alloy leaf in concentrated nitric acid. The obtained NPGF was attached onto glassy carbon electrode and then was functionalized with BPA-specific aptamer via the formation of Au-S bond. The fabrication of the sensor was characterized by scanning electron microscopy and X-ray photoelectron spectroscopy. NPGF exhibited excellent electrocatalytic activity towards the redox reaction of BPA, which ensured high sensitivity of the sensor. The aptamer-captured BPA showed a pair of redox peaks around 0.35/0.28 V (vs. Ag/AgCl). The experimental parameters in terms of aptamer concentration, reaction time, pH, and temperature were optimized. The calibration plot showed a linear range from 0.1 nM to 100 nM BPA with a remarkable detection limit of 0.056 +/- 0.004 nM BPA. Particularly, the successful application of the developed sensor for the detection of BPA in human serum samples suggests its promising potential for clinical diagnosis. (C) 2015 Elsevier B.V. All rights reserved.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:32
Scopus被引频次:37
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84931009583&doi=10.1016%2fj.aca.2015.05.002&partnerID=40&md5=34eb6fefb371366d15961e922c7be8c7
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