标题:Urinary trypsin inhibitor attenuates lipopolysaccharide-induced acute lung injury by blocking the activation of p38 mitogen-activated protein kinase.
作者:Zhang X;Liu F;Liu H;Cheng H;Wang W;Wen Q;Wang Y
作者机构:[Zhang, X] Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, Jinan 250021, China;[ Liu, F] Depart 更多
通讯作者:Wang, YL
通讯作者地址:[Wang, YL]Shandong Univ, Prov Hosp, Dept Pediat, 324 Jingwuweiqi Rd, Jinan 250021, Peoples R China.
来源:Inflammation research: Official journal of the European Histamine Research Society
出版年:2011
卷:60
期:6
页码:569-575
DOI:10.1007/s00011-010-0305-2
关键词:Urinary trypsin inhibitor; Lipopolysaccharide; Acute lung injury; Tumor; necrosis factor-alpha; Interleukin-10; p38 Mitogen-activated protein; kinase
摘要:OBJECTIVE: To investigate the protective effect of urinary trypsin inhibitor (UTI) in a rat model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying molecular mechanism. METHODS: Rats were randomly assigned into three groups: control group, LPS treatment group and LPS/UTI treatment group. The serum concentrations of tumor necrosis factor (TNF)-alpha and interleukin (IL)-10 were measured by ELISA. The expression of p38 mitogen-activated protein kinase (MAPK) in lung tissues was determined by Western blot analysis. RESULTS: Administration of UTI reduced the lung wet/dry weight ratio and ameliorated the tissue damage. In the LPS/UTI treatment group, levels of TNF-alpha were significantly lower than those in the LPS treatment group, while the levels of IL-10 were significantly higher than those in the LPS treatment group. Western blot analysis revealed that UTI inhibited the phosphorylation of p38 MAPK in lung tissues. CONCLUSIONS: UTI attenuates LPS-induced ALI, probably by adjusting the balance between proinflammatory and anti-inflammatory cytokines. The mechanism responsible for the decreased TNF-alpha expression may be related to the inhibitory effect of UTI on p38 MAPK activation.
收录类别:SCOPUS;SCIE
WOS核心被引频次:15
Scopus被引频次:15
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-79956224306&doi=10.1007%2fs00011-010-0305-2&partnerID=40&md5=109a6ad022e5b0e69d7bd890ab8d3d5f
TOP