标题:Investigating the Impact of Asp181 Point Mutations on Interactions between PTP1B and Phosphotyrosine Substrate
作者:Liu, Mengyuan; Wang, Lushan; Sun, Xun; Zhao, Xian
作者机构:[Liu, Mengyuan; Sun, Xun; Zhao, Xian] Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China.; [Wang, Lushan] Shandong Univ, State 更多
通讯作者:Sun, X
通讯作者地址:[Sun, X]Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China.
来源:SCIENTIFIC REPORTS
出版年:2014
卷:4
DOI:10.1038/srep05095
摘要:Protein tyrosine phosphatase 1B (PTP1B) is a key negative regulator of insulin and leptin signaling, which suggests that it is an attractive therapeutic target in type II diabetes and obesity. The aim of this research is to explore residues which interact with phosphotyrosine substrate can be affected by D181 point mutations and lead to increased substrate binding. To achieve this goal, molecular dynamics simulations were performed on wild type (WT) and two mutated PTP1B/substrate complexes. The cross-correlation and principal component analyses show that point mutations can affect the motions of some residues in the active site of PTP1B. Moreover, the hydrogen bond and energy decomposition analyses indicate that apart from residue 181, point mutations have influence on the interactions of substrate with several residues in the active site of PTP1B.
收录类别:SCOPUS;SCIE
WOS核心被引频次:20
Scopus被引频次:24
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901707653&doi=10.1038%2fsrep05095&partnerID=40&md5=214291599b77fa0a62bd12ef64e24157
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