标题：Development of the cellulolytic fungus Trichoderma reesei strain with enhanced β-glucosidase and filter paper activity using strong artifical cellobiohydrolase 1 promoter
作者：Zhang, J.;Zhong, Y.;Zhao, X.;Wang, T.
作者机构：[Zhang, J] State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;[ Zhong, Y] State Key Laboratory of Microbial Techno 更多
通讯作者地址：[Wang, TH]Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China.
来源：Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies
关键词：β-Glucosidase 1;Cellulase;Modified four-copy cbh1 promoter;Trichoderma reesei
摘要：To improve the β-glucosidase yield and total cellulase activity of Trichoderma reesei, extracellular β-glucosidase (BGLI) was overexpressed under the control of the modified four-copy cbh1 promoter. Three transformants B2, B12 and B15 with successful integration of the bgl1 gene expression cassette were obtained, which exhibited 3.7-, 2.0- and 1.8-fold increase in β-glucosidase activity than the parental strain RUT C30, respectively. The filter paper activities of the productive transformants B12 and B15 were improved by up to 130% and 55%, respectively. Saccharification of corncob residue with the crude enzyme dosages showed that the reducing sugar yields of B12 (5.59. mg/ml) and B15 (4.80. mg/ml) were 29% and 11% higher than that of RUT C30 (4.34. mg/ml), respectively. The present results proved that the modified four-copy cbh1 promoter was a useful tool for improving the cellulase activity of T. reesei, and the engineering strains developed in this study could be potentially used as promising cell factories for β-glucosidase or cellulase production.