标题：A facile method to prepare porous graphene with tunable structure as electrode materials for immobilization of glucose oxidase
作者：Wu, Haikun; Lu, Lu; Zhang, Yan; Sun, Zhongyu; Qian, Lei
作者机构：[Wu, Haikun; Lu, Lu; Zhang, Yan; Sun, Zhongyu; Qian, Lei] Shandong Univ, Minist Educ, Key Lab Liquid Solid Struct Evolut & Proc Mat, 17923 Jingshi Rd, 更多
通讯作者地址：[Qian, L]Shandong Univ, Minist Educ, Key Lab Liquid Solid Struct Evolut & Proc Mat, 17923 Jingshi Rd, Jinan 250061, Peoples R China.
来源：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
关键词：Porous graphene; Freeze drying; Electrode materials; Glucose oxidase
摘要：We first reported a facile method to prepare porous graphene with tunable structure for glucose oxidase (GOD) immobilization based on freeze-drying. The produced porous graphene (PGR) exhibited three-dimensional interconnected porous structure. Moreover, their microstructure was easily tuned by addition of surfactant and oil droplets into the graphene oxide dispersion. Field emission scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy and nitrogen physisorption were used to characterize microstructures, composition and surface area of the produced PGR. The addition of surfactant introduced mesopores in the PGR and the oil droplets can improve the pore volume. It was found that the PGR structure obviously affected the electrochemical behavior of GOD. Electrocatalytical activity towards glucose was also investigated, and the maximum electrocatalytical current reached 6.3 times for 5 mM glucose. The modified electrode was used to detect the glucose, and a low detection limit of 8.7 OA and a high sensitivity of 16.3 pAmM cm(-2) were obtained. The good sensing performance was attributed to the good three-dimensional porous structure and conductivity of the PGR. (C) 2016 Elsevier B.V. All rights reserved.