标题：Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System
作者：Li, Dianxiang; Liang, Yongli; Wang, Xianwei; Wang, Lei; Qi, Mei; Yu, Yang; Luan, Yuanyuan
作者机构：[Li, Dianxiang; Qi, Mei; Yu, Yang; Luan, Yuanyuan] Univ Jinan, Sch Biol Sci & Biotechnol, Dept Biotechnol, Jinan 250022, Peoples R China.; [Liang, Y 更多
通讯作者地址：[Li, DX]336 West Rd Nan Xinzhuang, Jinan 250022, Shandong, Peoples R China.
来源：G3-GENES GENOMES GENETICS
关键词：propheno-loxidase-activating system; gene identification; transcriptomic; analysis; Musca domestica; genetics of immunity
摘要：The proPO system regulates melanization in arthropods. However, the genes that are involved in the proPO system in housefly Musca domestica remain unclear. Thus, this study analyzed the combined transcriptome obtained from M. domestica larvae, pupae, and adults that were either normal or bacteria-challenged by an Escherichia coli and Staphylococcus aureus mixture. A total of 54,821,138 clean reads (4.93 Gb) were yielded by Illumina sequencing, which were de novo assembled into 89,842 unigenes. Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified. Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors. The expression levels of these identified genes were investigated by qRT-PCR assay, which were consistent with expected activation process of the proPO system, and their activation functions were confirmed by the measurement of phenoloxidase activity in bacteria-infected larvae after proPO antibody blockage, suggesting these candidate genes might have potentially different roles in the activation of proPO system. Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.