标题：Therapeutic effects of interleukin-37 and induced cardiosphere on treating myocardial ischemia-reperfusion injury
作者：Li J.; Zhang W.-J.; Yao H.; Li T.-M.
作者机构：[Li, J] Preventive Medicine Ward, Wei Hai Municpal Hospital, Shandong University, Weihai, Shandong 2642, China;[ Zhang, W.-J] Intensive Care Unit(ICU 更多
通讯作者地址：[Li, T.-M] Intensive Care Unit (ICU) of Wei Hai Municpal Hospital, No. 70, Heping Road, China;
关键词：iCS; IL-37; Induced cardiospheres; MI-RI; Myocardial ischemia-reperfusion injury
摘要：Backgrounds: Myocardial ischemia-reperfusion injury (MI-RI) has many adverse complications with high mortality rate. In the current study, we investigated the therapeutic advantages of delivering Interleukin-37 (IL-37) by induced cardiospheres (iCS), generated from adult skin fibroblasts via somatic reprogramming, in treating the mice model MI-RI. Methods: The mouse model of MI-RI was established and the iCS cells with IL-37 overexpression (iCS-IL37) were transplanted into the mice via tail-vein injection. Left ventricular (LV) dimensions and LV pressure-volume measurements were assessed by parasternal long-axis echocardiography and hemodynamic assessment. The infarct size was determined by histology analysis. And the inflammatory responses were analyzed by using enzyme-linked immunosorbent assay (ELISA). Results: The LV function was significantly improved after the iCS-IL37 transplantation when compared to the vehicle control group and iCS group, including the end-systolic pressure and dP/dtMax. Furthermore, the infarct size was significantly decreased after the iCS-IL37 transplantation. The protein levels of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), were down-regulated by the iCS-IL37 transplantation. Conclusion: The present study indicated that the iCS with IL-37 overexpression had therapeutic effects on the mice model of MI-RI. © 2020