标题：Chitosan Microsphere Used as an Effective System to Deliver a Linked Antigenic Peptides Vaccine Protect Mice Against Acute and Chronic Toxoplasmosis
作者：Guo, Jingjing; Sun, Xiahui; Yin, Huiquan; Wang, Ting; Li, Yan; Zhou, Chunxue; Zhou, Huaiyu; He, Shenyi; Cong, Hua
作者机构：[Guo, Jingjing; Sun, Xiahui; Yin, Huiquan; Wang, Ting; Li, Yan; Zhou, Chunxue; Zhou, Huaiyu; He, Shenyi; Cong, Hua] Shandong Univ, Sch Med, Dept Human 更多
通讯作者：He, SY;Cong, H
通讯作者地址：[He, SY; Cong, H]Shandong Univ, Sch Med, Dept Human Parasitol, Jinan, Shandong, Peoples R China.
来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
关键词：Toxoplasma gondii; chitosan microspheres; peptides; epitopes; vaccine
摘要：Multiple antigenic peptide (MAP) vaccines have advantages over traditional Toxoplasma gondii vaccines, but are more susceptible to enzymatic degradation As an effective delivery system, chitosan microspheres (CS) can overcome this obstacle and act as a natural adjuvant to promote T helper 1 (Th1) cellular immune responses. In this study, we use chitosan microparticles to deliver multiple antigenic epitopes from GRA10 (G10E), containing three dominant epitopes. When G10E was entrapped within chitosan microparticles (G10E-CS), adequate peptides for eliciting immune response were loaded in the microsphere core and this complex released G10E peptides stably. The efficiency of G10E-CS was detected both in vitro, via cell culture, and through in vivo mouse immunization. In vitro, G10E-CS activated Dendritic Cells (DC) and T lymphocytes by upregulating the secretion of eostimulatory molecules (CD40 and CD86). In vivo, Th1 biased cellular and humoral immune responses were activated in mice vaccinated with G10E-CS, accompanied by significantly increased production of IFN-gamma, IL-2, and IgG, and decreases in IL-4, IL-10, and lgG1. Immunization with G10E-CS conferred significant protection with prolonged survival in mice model of acute toxoplasmosis and statistically significant decreases in cyst burden in murine chronic toxoplasmosis. The results from this study indicate that chitosan microspheres used as an effective system to deliver a linked antigenic peptides is a promising strategy for the development of efficient vaccine against T. gondii.