标题：The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
作者：Li F.; Wang Y.; Yang H.; Xu Y.; Zhou X.; Zhang X.; Xie Z.; Bi J.
作者机构：[Li, F] Department of Neurology, Second Hospital of Shandong University, Shandong University, 247 North Park Avenue, Jinan, 250033, China;[ Wang, Y] D 更多
通讯作者地址：[Xie, Z] Department of Neurology, Second Hospital of Shandong University, Shandong University, 247 North Park Avenue, China;
来源：BMC Molecular Biology
关键词：Alzheimer's disease; Aβ; BACE1-AS; lncRNA
摘要：Background: The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1 mRNA and promotes the formation of Aβ. After the administration of Aβ 1-42 to SH-SY5Y cells and C57/BL6J mice, we detected the expression of BACE1-AS, BACE1 mRNA, and BACE1 protein, as well as the concentration of Aβ 1-40. Then, we silenced the expression of BACE1-AS in SH-SY5Y and 20E2 cells using siRNAs targeting BACE1-AS and detected its effects on the levels of the BACE1 mRNA and BACE1 protein and Aβ 1-40 generation. Results: The administration of Aβ 1-42 increased the expression of BACE1-AS, BACE1 mRNA and protein, as well as the concentration of Aβ 1-40 in SH-SY5Y cells and the brains of C57BL/6J mice. Pretreatment with the BACE1-AS siRNA inhibited the effect of Aβ 1-42 on increasing the expression of BACE1-AS and BACE1, as well as the generation of Aβ. Conclusions: The mechanism by which exogenous Aβ 1-42 induces BACE1 expression and Aβ generation is mediated by BACE1-AS. BACE1-AS is involved in the mechanism regulating BACE1 expression and Aβ generation in APPsw transgenic cells. © 2019 The Author(s).