标题：Glyceraldehyde-3-Phosphate Dehydrogenase of Babesia microti Is a Plasminogen- and Actin-Binding Protein
作者：Liu, Xiangye; Li, Huiqin; Deng, Hongkuan; Zheng, Chen; Yan, Hongru; Chen, Zetian; Bian, Anning; Chen, Jiaxu; Zheng, Kuiyang
作者机构：[Liu, Xiangye; Li, Huiqin; Zheng, Chen; Yan, Hongru; Chen, Zetian; Bian, Anning; Zheng, Kuiyang] Xuzhou Med Univ, Jiangsu Key Lab Immun & Metab, Dept 更多
通讯作者地址：[Liu, XY]Xuzhou Med Univ, Jiangsu Key Lab Immun & Metab, Dept Pathogen Biol & Immunol, Xuzhou, Jiangsu, Peoples R China.
来源：FRONTIERS IN VETERINARY SCIENCE
关键词：Babesia microti; glyceraldehyde-3-phosphate dehydrogenase; plasminogen;; alpha-actin; binding protein
摘要：Babesia microti, an intraerythrocytic protozoa, can cause an emerging tick-borne disease-Human babesiosis. The parasite can successfully invade host red blood cells owing to the assistance of molecules expressed by babesia. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the housekeeping intracellular glycolytic enzyme, can also be expressed in the external of cells, where contributes to binding to several molecules such as plasminogen and actin. In the present study, we identified B. microti GAPDH (BmGAPDH) and generated the recombinant BmGAPDH (rBmGAPDH) via an E.coli expression system. Furthermore, we confirmed its catalytic dehydration activity in vitro. Moreover, we also demonstrated that rBmGAPDH could bind to human plasminogen and mouse alpha-actin. In addition, we demonstrated that rBmGAPDH could recognize anti-B. microti mouse serum. In conclusion, BmGAPDH is a multifunctional glycolytic enzyme, which can bind to host plasminogen and alpha-actin.