标题：Versatile enzymatic assays by switching on the fluorescence of gold nanoclusters
作者：Sun, Dongxue; Li, Ping; Liu, Qingyun; Liu, Tianli; Gu, Mengmeng; Wang, Guang-Li
作者机构：[Sun, Dongxue; Li, Ping; Liu, Tianli; Gu, Mengmeng; Wang, Guang-Li] Jiangnan Univ, Minist Educ, Key Lab Synthet & Biol Colloids, Int Joint Res Ctr Pho 更多
通讯作者：Wang, GuangLi;Wang, GL
通讯作者地址：[Wang, GL]Jiangnan Univ, Minist Educ, Key Lab Synthet & Biol Colloids, Int Joint Res Ctr Photorespons Mol & Mat,Sch Chem, Wuxi 214122, Jiangsu, People 更多
来源：ANALYTICA CHIMICA ACTA
关键词：Gold nanoclusters; Signal-on detection; Enzymatic bioassays
摘要：Herein we present a general and turn-on strategy for enzymatic bioassays on the basis of redox state dependent emission of gold nanoclusters (AuNCs). The photoluminescence of AuNCs was quenched obviously by the oxidative ferricyanide while unaffected by its corresponding reduced state, i.e., ferrocyanide. The distinctive quenching abilities for AuNCs by the redox couple (ferricyanide/ferrocyanide) enabled their utility as new fluorescent sensing platforms to detect redox-related phenomena. The proposed protocols were conducted by using the model oxidoreductases of glucose oxidase (GOx) and the enzyme cascade of lactate dehydrogenase (LDH)/diaphorase to catalytically convert ferricyanide to ferrocyanide, which switched on fluorescence of the detection systems. The detection limit for glucose and lactate was found to be as low as 0.12 and 0.09 mu M, respectively. This work features the first use of the redox couple of ferricyanide/ferrocyanide in fluorescent bioanalysis, which enables versatile, signal on and highly sensitive/selective detections as compared to the state of the art fluorescently enzymatic sensing platforms. Importantly, considering the significance of ferricyanide/ferrocyanide involves in numerous other oxidoreductases mediated biocatalysis, this protocol has wide versatility that enables combination with oxidoreductases related reactions for biosensing. (C) 2019 Elsevier B.V. All rights reserved.