标题：Voluntary exercise training attenuated the middle-aged maturity-induced cardiac apoptosis
作者：Cui J.-W.; Hong Y.; Kuo Y.-M.; Yu S.-H.; Wu X.-B.; Cui Z.-Y.; Lee S.-D.
作者机构：[Cui, J.-W] School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China, Institute of Rehabilitation Medici 更多[Cui, J.-W] School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China, Institute of Rehabilitation Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China;[ Hong, Y] The First Rehabilitation Hospital of Shanghai, Shanghai, China;[ Kuo, Y.-M] Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan;[ Yu, S.-H] College of Rehabilitation, Shandong University of Traditional Chinese MedicineShandong, China;[ Wu, X.-B] School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China, Departmental of Rehabilitation, Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China;[ Cui, Z.-Y] School of Rehabilitation Medicine, Weifang Medical UniversityShandong, China;[ Lee, S.-D] School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China, College of Rehabilitation, Shandong University of Traditional Chinese MedicineShandong, China, Departmental of Rehabilitation, Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China, School of Rehabilitation Medicine, Weifang Medical UniversityShandong, China, Department of Physical Therapy, Asia University, Taichung, Taiwan, Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan 收起 通讯作者：Lee, SD(shinda@mail.cmu.edu.tw)
通讯作者地址：[Lee, S.-D] Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, 91 Hsueh-Shih Road, Taiwan;
来源：Life Sciences
出版年：2020
卷：259
DOI：10.1016/j.lfs.2020.118187
关键词：Caspase-independent; Cell death; Fas dependent; IGF-related; Mitochondrial
摘要：Aims: Voluntary exercise training has cardioprotective effects in humans, but the underlying mechanism is unknown. This research was done to estimate the effect of voluntary exercise training to attenuate middle-aged maturity-induced cardiac apoptosis. Materials and methods: The study was designed to divide 64 male mice randomly into four groups, consisting of a 9-month sedentary pre-middle-aged group (9M), 15-month sedentary middle-aged group (15M), and two exercise groups using a voluntary wheel running respectively (9M+EX, 15M+EX). After 3 months, the condition of cardiac apoptosis in different groups was measured by HE dying, TUNEL and DAPI staining, and Western Blot analysis. Key findings: TUNEL-positive cells were increased in 15M group compared with 9M group, while decreased in 9M+EX and 15M+EX groups compared with their control groups respectively. Protein levels of AIF, Endo G, TNF-α, TNFR1, TRAF2, TRADD, Fas, FasL, FADD, activated caspase 8, 3, 9, Bax/Bcl2, Bak/BclxL, and tBid were decreased in 9M+EX and 15M+EX groups compared with their control groups respectively. The protein levels of pBad/Bad, 14-3-3, IGF1, IGFR1, pPI3K/PI3K, and pAKT/AKT were more activated in the 9M+EX and 15M+EX groups than those in their control groups respectively. Significant differences were found between 9M group and 15M group for the protein levels of TRAF2, FADD, Bax/Bcl2, tBid and pAKT/AKT. Significance: Voluntary exercise training as an important lifestyle modification may prevent cardiac widely dispersed apoptosis and enhance cardiac survival at middle-aged maturity. © 2020 Elsevier Inc.
收录类别：SCOPUS
资源类型：期刊论文
原文链接：https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089800439&doi=10.1016%2fj.lfs.2020.118187&partnerID=40&md5=33679e39486d6f7867e6beaa078a92dd
