标题：Immunoassay for carcinoembryonic antigen based on the Zn+2-enhanced fluorescence of magnetic-fluorescent nanocomposites
作者：Wu, Kaiqing; Chu, Chengchao; Ma, Chao; Yang, Hongmei; Yan, Mei; Ge, Shenguang; Yu, Jinghua; Song, Xianrang
作者机构：[Wu, Kaiqing; Chu, Chengchao; Ma, Chao; Yang, Hongmei; Yu, Jinghua] Univ Jinan, Shandong Univ, Sch Chem & Chem Engn, Key Lab Chem Sensing & Anal, Jina 更多
通讯作者地址：[Yu, JH]Univ Jinan, Shandong Univ, Sch Chem & Chem Engn, Key Lab Chem Sensing & Anal, Jinan 250022, Peoples R China.
来源：SENSORS AND ACTUATORS B-CHEMICAL
关键词：Magnetic composite nanoparticle; ZnO nanowires; Fluorescence intensity; enhanced; Fluorescent immunoassay
摘要：Carbon coated magnetic composite nanoparticles (Fe3O4@C) were prepared and further fabricated into a new kind of the multifunctional core/shell nanoparticles (Fe3O4@C@CdTe) by a self-assembly of CdTe quantum dots. These nanoparticles exhibited good fluorescence (FL) property. In addition, zinc oxide (ZnO) nanowires (NWs) with high surface area were synthesized via solvothermal reaction under a hydrothermal condition. In a sandwich immune-device for the detection of carcinoembryonic antigen (CEA), the Fe3O4@C@CdTe nanoparticles were applied as the solid support for CEA-primary monoclonal antibody (CEA-McAb(1)) and the ZnO NWs were applied as the label of CEA-secondary monoclonal antibody (CEA-McAb(2)). The Fe3O4@C@CdTe-McAb(1) was collected at the bottom of the quartz cuvette using a magnet. The ZnO NWs bond with Fe3O4@C@CdTe after the immune reaction. The Zn2+ ions are subsequently released from ZnO NWs by dissolution in acidic buffer solution and the enhanced FL intensity is measured. In this way, the proposed FL immune-device displayed excellent analytical performance for the detection of CEA in the range of 0.005-100 ngmL(-1) with a low detection limit of 1.6 pgmL(-1), and the assay results of CEA in real serum samples were in a good agreement with the standard enzyme-linked immunosorbent assay (ELISA) tests. (C) 2014 Elsevier B.V. All rights reserved.