标题：Danshensu-mediated protective effect against hepatic fibrosis induced by carbon tetrachloride in rats
作者：Qu, W.; Huang, H.; Li, K.; Qin, C.
作者机构：[Qu, W.; Qin, C.] Shandong Univ, Prov Hosp, Dept Gastroenterol, Jinan 250021, Shandong, Peoples R China.; [Qu, W.] Cent Hosp Taian, Dept Nosocomial 更多
通讯作者地址：[Qin, C]Shandong Univ, Prov Hosp, Dept Gastroenterol, 324 Jingwuweiqi Rd, Jinan 250021, Shandong, Peoples R China.
关键词：Danshensu; Carbon tetrachloride; Hepatic fibrosis; Metabolism;; Hepatoprotection
摘要：The culprit of hepatic fibrosis (HF) is linked to suprathreshold deposition of collagen. Thus, collagen reduction by improved metabolism contributes to HF management. In this study, we aimed to investigate the hepatoprotective effects of Danshensu (DSS) against carbon tetrachloride (CCl4)-induced HF rats. The results showed that DSS-administrated rats resulted in decreasing in hepatosomatic indexes, and lowering serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Meanwhile, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) were increased, while the content of malonaldehyde (MDA) was lessened in liver tissue of DSS administration group. In addition, the pro-fibrotic markers of hydroxyproline (Hyp), type III procollagen (PCIII) and hyaluronic acid (HA) contents were decreased. Histopathological examination confirmed that the hepatotoxicity in CCl4-injured rats was alleviated following the DSS administration. Furthermore, intrahepatic protein expressions of alpha-smooth muscle actin (alpha-SMA), phosphorylated JAK2 (p-JAK2) and phosphorylated STAT3 (p-STAT3) were effectively down-regulated, respectively. Overall, this work demonstrates that DSS played the protective effect against CCl4-induced cytotoxicity in liver tissue, which the probable mechanism is associated with attenuation of lipid peroxidation, collagen accumulation and enhancement of anti-oxidative defense capability, as well as regulation of intrahepatic JAK/STAT pathway for maintaining collagenic homoeostasis. (C) 2014 Elsevier Masson SAS. All rights reserved.