标题：MicroRNA-21 promotes bone reconstruction in maxillary bone defects
作者：Wang H.; Li X.; Zhang Z.; Zhao X.; Wang C.; Wei F.
作者机构：[Wang, H] Department of Orthodontics, School and Hospital of Stomatology, Shandong University, Jinan, China, Shandong Provincial Key Laboratory of Ora 更多
通讯作者地址：[Wang, C] Department of Orthodontics, School and Hospital of Stomatology, Shandong UniversityChina;
来源：Journal of Oral Rehabilitation
关键词：bone defect; bone reconstruction; maxilla; maxillary rehabilitation; microRNA-21; microRNAs
摘要：Background: Bone reconstruction of the maxillary bone defects is an urgent issue due to its functional and aesthetic influence. MicroRNAs (miRNAs) are a class of non-coding RNAs that function in diverse biological and pathological processes. Recently, microRNA-21 (miR-21) was reported to play significant roles in bone formation, suggesting that miR-21 can be novel biomarker and therapeutic target for bone remodelling and skeletal diseases. However, the role of miR-21 in maxillary bone defects remains unclear. Objective and Methods: This study aimed to investigate the effect of miR-21 on the bone reconstruction by inducing maxillary bone defects in wild-type (WT) and miR-21 knockout (miR-21-KO) mice and explore these mice as maxillary bone defect models. Results: Micro-computed tomography (micro-CT) and histochemistry showed that the miR-21-KO mice had reduced bone reformation ability compared with the WT mice. The expression levels of alkaline phosphatase (ALP) and osteocalcin (OCN) were dramatically decreased in the miR-21-KO mice. In addition, injection of miR-21 agomir intra-peritoneally into miR-21-KO mice (miR-21-KO+ agomir) following the maxillary bone defects surgery displayed a significantly enhanced bone formation -promoting ability, which indicated that miR-21 agomir could ameliorate maxillary bone defects in miR-21-KO mice in vivo. Furthermore, immunohistochemistry suggested that ALP and OCN expressions were prominently increased in miR-21-KO+ agomir mice. Conclusion: These findings demonstrated that miR-21 deficiency impaired bone reformation and miR-21 contributed to the bone reconstruction of the maxillary bone defects. The evidence also supported the use of WT and miR-21-KO mice as maxillary bone defect models for further research. © 2019 John Wiley & Sons Ltd