标题:Suppression of toll-like receptor 2 expression inhibits the bioactivity of human hepatocellular carcinoma.
作者:Wenna Shi;Lihui Su;Qianqian Li;Lidan Sun;Jing Lv;Jun Li;Baoquan Cheng
作者机构:[Shi, W] Department of Gastroenterology, Qilu Hospital, School of Medicine, Shandong University, 107#, Wenhua Xi Road, Jinan, 250012, China;[ Lihui, S 更多
通讯作者:Cheng, BQ
通讯作者地址:[Cheng, BQ]Shandong Univ, Sch Med, Qilu Hosp, Dept Gastroenterol, 107 Wenhua Xi Rd, Jinan 250012, Peoples R China.
来源:Tumour biology: the journal of the International Society for Oncodevelopmental Biology and Medicine
出版年:2014
卷:35
期:10
页码:9627-9637
DOI:10.1007/s13277-014-2268-3
关键词:Hepatocellular carcinoma; Toll-like receptor 2; High mobility group; box1; Nuclear factor-kappaB/P65; Apoptosis
摘要:Toll-like receptor (TLR) 2 signaling is regarded as one of the mechanisms of chronic inflammation, but it can also mediate tumor cell immune escape and tumor progression. However, the role of TLR2 in the progression of human hepatocellular carcinoma (HCC) remains unclear. The objective of the study was to examine the effect of TLR2 on the bioactivity of HCC cell lines, HepG2 and BEL-7402, and the relationship between high mobility group box1 (HMGB1) and TLR2. The expression of TLR2 and nuclear factor-kappaB/P65 (NF-κB/P65) in HepG2 and BEL-7402 was assayed by Western blot. Cells were transfected with specific small interfering RNAs of TLR2 (TLR2-siRNAs), then TLR2-siRNA-transfected cells were treated with recombinant HMGB1 (rHMGB1). Apoptosis was determined by flow cytometry. Results showed that TLR2 was expressed in HepG2 and BEL-7402 cells. The ability of proliferation, invasion, and migration in siRNA group was lower than that in blank group, and the apoptosis ratio was higher than that in blank group, respectively. NF-κB/P65 expression was declined in contrast with blank group. Downregulation of TLR2 by siRNA resulted in a significant inhibition of proliferation, invasion, migration, and NF-κB/P65 expression, and elevated apoptotic ratio. Conversely, rHMGB1 promoted proliferation, invasion, and migration, induced NF-κB/P65 expression, and inhibited cells apoptosis. Furthermore, downregulation of TLR2 weakened the role of rHMGB1. This study suggests TLR2 and HMGB1 are important targets for therapeutic intervention of HCC.
收录类别:SCOPUS;SCIE
WOS核心被引频次:11
Scopus被引频次:11
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84922392512&doi=10.1007%2fs13277-014-2268-3&partnerID=40&md5=cc6264a530e69a66136d5ff696a5e4fb
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