标题:Electrochemical immunoassay for carcinoembryonic antigen based on signal amplification strategy of nanotubular mesoporous PdCu alloy
作者:Cai, Yanyan; Li, He; Li, Yuyang; Zhao, Yanfang; Ma, Hongmin; Zhu, Baocun; Xu, Caixia; Wei, Qin; Wu, Dan; Du, Bin
作者机构:[Cai, Yanyan; Li, He; Li, Yuyang; Zhao, Yanfang; Ma, Hongmin; Zhu, Baocun; Xu, Caixia; Wei, Qin; Wu, Dan; Du, Bin] Univ Jinan, Shandong Univ, Sch Chem 更多
通讯作者:Wei, Q
通讯作者地址:[Wei, Q]Univ Jinan, Shandong Univ, Sch Chem & Chem Engn, Key Lab Chem Sensing & Anal, Jinan 250022, Peoples R China.
来源:BIOSENSORS & BIOELECTRONICS
出版年:2012
卷:36
期:1
页码:6-11
DOI:10.1016/j.bios.2012.02.064
关键词:Label-free immunosensor; Hollow nanostructure; Graphene;; Carcinoembryonic antigen; Thionine
摘要:Interests in using nanoporous metals for biosensing applications have been increasing. Herein, nanotubular mesoporous PdCu (NM-PdCu) alloy is used to fabricate a novel label-free electrochemical immunosensor for cancer biomarker carcinoembryonic antigen (CEA). It operates through physisorption of anti-CEA on NM-PdCu and the mixture of sulfonated graphene sheets (HSO3-GS) and thionine (TH) functionalized glassy carbon electrode interface as the detection platform. In this study, chitosan (CS)-PdCu is bound very strongly to carcinoembryonic antibody (anti-CEA), because of the good electron conductivity, high surface area, and good biocompatibility. CS-PdCu is immobilized on electrodes by electrostatic interactions between the negatively charged sulfo group of HSO3-GS and the abundant positively charged amino groups of chitosan. TH acts as the redox probe. Under the optimized conditions, the electrochemical immunosensor exhibits a wide working range from 0.01 to 12 ng/mL with a low detection limit of 4.86 pg/mL. The accuracy, reproducibility, and stability of the immunosensor are acceptable. The assay is evaluated for real serum samples, receiving satisfactory results. The nanoporous metal materials-based immunoassay provides a promising approach in clinical application and thus represents a versatile detection method. (C) 2012 Elsevier B.V. All rights reserved.
收录类别:EI;SCIE
WOS核心被引频次:39
资源类型:期刊论文
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