标题：Pathogenesis analysis of pituitary adenoma based on gene expression profiling
作者：Wang, Weimin;Xu, Zhiming;Fu, Li;Liu, Wei;Li, Xingang
作者机构：[Wang, W] School of Medicine, Shandong University, Jinan, Shandong 250012, China, Department of Neurosurgery, Qingdao Municipal Hospital, Qingdao, Sh 更多
通讯作者地址：[Li, XG]Shandong Univ, Sch Med, 44 Wenhua Xi Rd, Jinan 250012, Shandong, Peoples R China.
关键词：pituitary adenoma;differentially-expressed genes;interleukin 6;signal transducer and activator of transcription 3;POU1F1
摘要：The aim of the current study was to investigate the pathogenesis of pituitary-adenoma through screening of the differentially-expressed genes (DEGs) and proteins in normal pituitary and pituitary adenoma tissues, and analyzing the interactions among them. Following the acquisition of gene expression profiling data from a public functional genomics data repository, Gene Expression Omnibus, DEGs were screened in normal pituitary and pituitary adenoma tissues. Upregulated and downregulated DEGs were further identified through gene ontology functional enrichment analysis. Subsequently, the DEGs were mapped to the Search Tool for the Retrieval of Interacting Genes database, and the protein-protein interaction (PPI) networks of the upregulated and downregulated DEGs were constructed. Finally, the functional modules of the PPI network of the downregulated DEGs were analyzed. In total, 211 upregulated and 413 downregulated DEGs were screened between the normal pituitary and pituitary adenoma samples. Downregulated DEGs were associated with certain functions, including the immune response, hormone regulation and cell proliferation. Upregulated genes were associated with cation transport functions. Five modules were acquired from the PPI network of the downregulated DEGs. Transcription factors, including signal transducer and activator of transcription 3 (STAT3), interleukin 6 (IL-6), B-cell lymphoma 6 protein, early growth response 1, POU1F1, jun B proto-oncogene and FOS were the core nodes in the functional modules. In summary, the DEGs and proteins were identified through screening gene expression profiling and PPI networks. The results of the present study indicated that low expression levels of hormone- and immune-related genes facilitated the occurrence of pituitary adenoma. Low expression levels of IL-6 and STAT3 were significant in the dysimmunity of pituitary adenoma. Furthermore, the low expression level of POI1F1 contributed to the reduction in pituitary hormone se