标题：Clinical Implications of Evolutionary Patterns of Homologous, Full-Length Hepatitis B Virus Quasispecies in Different Hosts after Perinatal Infection
作者：Liu, Feng; Yu, De-Min; Huang, Su-Yuan; Yu, Jia-Lun; Zhang, Dong-Hua; Gong, Qi-Ming; Zhang, Xin-Xin
作者机构：[Liu, Feng; Yu, De-Min; Huang, Su-Yuan; Yu, Jia-Lun; Zhang, Dong-Hua; Gong, Qi-Ming; Zhang, Xin-Xin] Shanghai Jiao Tong Univ, Sch Med, Inst Infect & R 更多
通讯作者地址：[Gong, QM]Shanghai Jiao Tong Univ, Sch Med, Inst Infect & Resp Dis, Dept Infect Dis,Ruijin Hosp, Shanghai 200030, Peoples R China.
来源：JOURNAL OF CLINICAL MICROBIOLOGY
摘要：Chronic hepatitis B virus (HBV) infection via perinatal transmission is common in the Asia-Pacific region, but related quasispecies (QS) characteristics are not yet defined. To investigate the homologous, full-length HBV QS after perinatal infection and their clinical implications, five pairs of mother-daughter patients with chronic HBV infection (one patient with liver cirrhosis, one with immune tolerance, and eight with chronic hepatitis) were included. Full-length HBV were amplified by PCR from serum samples before antiviral treatment and cloned; an average of 17 clones per sample were sequenced, and the QS complexities, diversities, and evolution patterns were analyzed. Full-length HBV sequence similarities within mother-daughter pairs were 91.3 to 98.3%. The QS complexities of full-length HBV were similar between mothers and daughters (median of 0.9734 compared to 0.9688, P > 0.05), as were the diversities (median of 3.396 x 10(-3) compared to 4.617 x 10(-3) substitutions/site, P > 0.05). However, the distribution patterns of QS complexities and diversities within specific genes were different, and QS genetic distances of the mothers were higher than those of daughters, both more evident in pairs with different antiviral responses and different immune phases or stages. The nucleotide substitution rate of full-length HBV was 14.388 x 10(-5) substitutions/site/year, whereas the preC/C gene rate was the highest. Mutations and indels were more common in clones from the mothers, which decreased the affinity of epitopes by 6- to 89- fold. The various genes from homologous HBV genomes evolved in different patterns despite numerically comparable full-length QS complexities and diversities. The different patterns may correlate with the immune stages of chronic HBV infection, which warrants further study.