标题：Metabolic engineering for improving l-tryptophan production in Escherichia coli
作者：Niu H.; Li R.; Liang Q.; Qi Q.; Li Q.; Gu P.
作者机构：[Niu, H] School of Biological Science and Technology, University of Jinan, Jinan, 250022, China;[ Li, R] School of Biological Science and Technology, 更多
通讯作者地址：[Gu, P] School of Biological Science and Technology, University of JinanChina;
来源：Journal of Industrial Microbiology and Biotechnology
关键词：Escherichia coli; l-Tryptophan; Metabolic engineering
摘要：l-Tryptophan is an important aromatic amino acid that is used widely in the food, chemical, and pharmaceutical industries. Compared with the traditional synthetic methods, production of l-tryptophan by microbes is environmentally friendly and has low production costs, and feed stocks are renewable. With the development of metabolic engineering, highly efficient production of l-tryptophan in Escherichia coli has been achieved by eliminating negative regulation factors, improving the intracellular level of precursors, engineering of transport systems and overexpression of rate-limiting enzymes. However, challenges remain for l-tryptophan biosynthesis to be cost-competitive. In this review, successful and applicable strategies derived from metabolic engineering for increasing l-tryptophan accumulation in E. coli are summarized. In addition, perspectives for further efficient production of l-tryptophan are discussed. © 2018, Society for Industrial Microbiology and Biotechnology.