标题:Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae
作者:Li, Jing; Wang, Xiao; Liu, Juan; Wang, Hong; Zhang, Xiao-Li; Tang, Wei; Sun, Yun-Dong; Wang, Xin; Yu, Xiu-Ping; Zhao, Wei-Ming
作者机构:[Li, Jing; Liu, Juan; Wang, Hong; Zhang, Xiao-Li; Tang, Wei; Sun, Yun-Dong; Wang, Xin; Yu, Xiu-Ping; Zhao, Wei-Ming] Shandong Univ, Sch Med, Dept Med 更多
通讯作者:Zhao, WM
通讯作者地址:[Zhao, WM]Shandong Univ, Sch Med, Dept Med Microbiol, Jinan 250012, Shandong, Peoples R China.
来源:VIROLOGY JOURNAL
出版年:2010
卷:7
DOI:10.1186/1743-422X-7-368
摘要:Background: To establish a convenient system for the study of human papillomavirus (HPV), we inserted a Saccharomyces cerevisiae selectable marker, Ura, into HPV58 genome and transformed it into yeast.; Results: HPV58 genome could replicate extrachromosomally in yeast, with transcription of its early and late genes. However, with mutation of the viral E2 gene, HPV58 genome lost its mitotic stability, and the transcription levels of E6 and E7 genes were upregulated.; Conclusions: E2 protein could participate in viral genome maintenance, replication and transcription regulation. This yeast model could be used for the study of certain aspects of HPV life cycle.
收录类别:SCOPUS;SCIE
WOS核心被引频次:1
Scopus被引频次:1
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-78650074937&doi=10.1186%2f1743-422X-7-368&partnerID=40&md5=8ff1fa3a9d88f3aab24e13d71935c88e
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