标题：Protective effect of calpeptin on acrylamide-induced microtubule injury in sciatic nerve
作者：Guan, Qiangdong; Su, Benyu; Wei, Xiaomin; Wang, Shue; Wang, Miaomiao; Liu, Ning; Jiang, Wenchong; Xu, Mengmeng; Yu, Sufang
作者机构：[Guan, Qiangdong; Su, Benyu; Wei, Xiaomin; Wang, Shue; Liu, Ning; Jiang, Wenchong; Xu, Mengmeng; Yu, Sufang] Shandong Univ, Sch Publ Hlth, 44 Wenhua X 更多
通讯作者地址：[Yu, SF]Shandong Univ, Sch Publ Hlth, 44 Wenhua Xi Rd, Jinan 250012, Shandong, Peoples R China.
关键词：Acrylamide; Calpain; Calpeptin; Microtubules; Sciatic nerve
摘要：The present study aimed to investigate the protective effect and mechanism of calpeptin (CP) on acrylamide (ACR)-induced microtubule (MT) injury in the sciatic nerve of rats. All rats were divided into four groups (control, CP, ACR, and ACR + CP):1 ml/kg saline, 200 mu g/kg CP, 30 mg/kg ACR, and 30 mg/kg ACR plus 200 mu g/kg CP were administered to the corresponding rats for 4 weeks through intraperitoneal injection. Body weight and neurobehavioral indicators were measured weekly and alpha-tubulin, beta-tubulin, and other concerned proteins were estimated by western blotting and immunohistochemistry. At 4 weeks, decreased body weight, increased gait scores, increased hindlimb splay, and decreased time of fall of ACR rats were observed compared with those of control rats. All these mentioned changes were restored in the ACR + CP group compared with the ACR group. After 4 weeks of administration, western blotting and immunohistochemistry revealed significant increase in the protein levels of beta-tubulin, calpain I, calpain II, Tau, microtubule-associated protein 2 (MAP2), protein kinase C, and cyclin-dependent kinase 5 in the ACR group compared with the control group; these increases were significantly lower in the ACR + CP group than in the ACR group. Furthermore, histopathological examination revealed loose arrangement, disorganised structure, uneven density, and exfoliated perineurium in the ACR group, and CP administration improved these changes significantly. The present results suggest that CP has an intervening effect on ACR-induced MT injury. A possible mechanism is that calpain maintains the stability of MTs by regulating the metabolism of Tau and MAP2.