标题：Exosomes Isolated From Human Umbilical Cord Mesenchymal Stem Cells Alleviate Neuroinflammation and Reduce Amyloid-Beta Deposition by Modulating Microglial Activation in Alzheimer's Disease
作者：Ding, Mao; Shen, Yang; Wang, Ping; Xie, Zhaohong; Xu, Shunliang; Zhu, ZhengYu; Wang, Yun; Lyu, Yongtao; Wang, Dewei; Xu, Linlin; B 更多 作者机构：[Ding, Mao; Shen, Yang] Shandong Univ, Sch Med, Jinan 250012, Shandong, Peoples R China.; [Wang, Ping; Xie, Zhaohong; Xu, Shunliang; Zhu, ZhengYu; W 更多
通讯作者：Bi, JZ;Yang, H
通讯作者地址：[Bi, JZ; Yang, H]Shandong Univ, Hosp 2, Dept Med Neurol, Jinan 250033, Shandong, Peoples R China.
关键词：Alzheimer's disease; Exosomes; Human umbilical cord mesenchymal stem; cells; Neuroinflammation; Microglial activation; Amyloid- peptides
摘要：Alzheimer's disease (AD) is the most common neurodegenerative disease characterized by excessive accumulation of the amyloid- peptide (A) in the brain, which has been considered to mediate the neuroinflammation process. Microglial activation is the main component of neuroimmunoregulation. In recent years, exosomes isolated from human umbilical cord mesenchymal stem cells (hucMSC-exosomes) have been demonstrated to mimic the therapeutic effects of hucMSCs in many inflammation-related diseases. In this study, exosomes from the supernatant of hucMSCs were injected into AD mouse models. We observed that hucMSC-exosomes injection could repair cognitive disfunctions and help to clear A deposition in these mice. Moreover, we found that hucMSC-exosomes injection could modulate the activation of microglia in brains of the mice to alleviated neuroinflammation. The levels of pro-inflammatory cytokines in peripheral blood and brains of mice were increased and the levels of anti-inflammatory cytokines were decreased. We also treated BV2 cells with hucMSC-exosomes in culture medium. HucMSC-exosomes also had inflammatory regulating effects to alternatively activate microglia and modulate the levels of inflammatory cytokines in vitro.