标题：Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway
作者：Wang, Jijun; Wang, Xiaolong; Chen, Tong; Jiang, Liyu; Yang, Qifeng
作者机构：[Wang, Jijun; Wang, Xiaolong; Jiang, Liyu; Yang, Qifeng] Shandong Univ, Qilu Hosp, Dept Breast Surg, Jinan, Shandong, Peoples R China.; [Chen, Tong] 更多
通讯作者：Jiang, LY;Yang, QF
通讯作者地址：[Jiang, LY; Yang, QF]Shandong Univ, Qilu Hosp, Dept Breast Surg, Jinan, Shandong, Peoples R China.
来源：CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
关键词：Huaier; H19; MiR-675-5p; CBL; Breast cancer
摘要：Background/Aims: Increasing evidence indicates that Huaier extract has promising therapeutic effects against cancer. However, the mechanisms that underlie its anti-tumor effects remain unclear. In recent years, various studies have shown that long noncoding RNAs (lncRNAs) play a critical role in the regulation of cancer development and progression. Here, we explored the role of lncRNAs in Huaier-induced tumor suppression. Methods: Microarray profiling was performed to identify the candidate lncRNAs affected by Huaier extract. Quantitative real-time PCR (qPCR) was used to evaluate the transfection efficiency and the influence of Huaier extract on H19 expression. The effect of Huaier extract on the cell viability was examined by MTT. Moreover, the rates of apoptotic cells were detected using flow-cytometric analysis. Western blot analysis was applied to show the protein levels of CBL. Results: Microarray data derived from Huaier-treated breast cancer cells identified H19 as a potential target. Huaier extract reduced the expression of H19. The over-expression of H19 inhibited the cytotoxic effects of Huaier extract; in contrast, reduced H19 expression enhanced the function of Huaier extract. MiR-675-5p was identified as a mature product of H19. Moreover, Huaier extract reduced the miR-675-5p expression. Upregulating miR-675-5p reversed the inhibitory effects of Huaier extract, whereas downregulating miR-675-5p sensitized breast cancer cells to the effect of Huaier extract. In addition, Huaier extract increased the expression of CBL protein, a direct target of miR-675-5p. Conclusion: Collectively, the data demonstrate that Huaier extract reduces viability and induces apoptosis in breast cancer cells via H19-miR-675-5p-CBL axis regulation. (C) 2017 The Author(s) Published by S. Karger AG, Basel