标题:Wnt/β-Catenin Signaling Pathway Upregulates c-Myc Expression to Promote Cell Proliferation of P19 Teratocarcinoma Cells
作者:Zhang,S.;Li,Y.;Wu,Y.;Shi,K.;Bing,L.;Hao,J.
作者机构:[Zhang, S] Department of Histology and Embryology, School of Medicine, Shandong University, Jinan 250012, China;[ Li, Y] Department of Histology and E 更多
通讯作者:Hao, J
通讯作者地址:[Hao, J]Shandong Univ, Sch Med, Dept Histol & Embryol, Jinan 250012, Peoples R China.
来源:The anatomical record: advances in integrative anatomy and evolutionary biology
出版年:2012
卷:295
期:12
页码:2104-2113
DOI:10.1002/ar.22592
关键词:β-catenin;C-myc;Embryonic carcinoma cells;Proliferation;Teratocarcinoma
摘要:Aberrant activation of the Wnt/β-catenin signaling pathway is a common event in human tumor progression. Wnt signaling has also been implicated in maintaining a variety of adult and embryonic stem cells by imposing a restraint to differentiation. To understand the function and mechanism of Wnt/β-catenin signaling on the pathogenesis of teratocarcinoma, we used the mouse teratocarcinoma P19 cell line as a model in vitro. Gsk3β specific inhibitor (SB216763) was used to activate Wnt/β-catenin signaling. All trans-retinoic acid (RA) was used to induce P19 cell differentiation. At different culture times, gene expression was examined by immunofluorescence staining, quantitative real-time PCR, and Western-blotting; BrdU incorporation assays were performed to measure P19 cell proliferation. Small interference RNA technology was used to downregulate c-myc expression. The results showed that SB216763 induced the nuclear translocation of β-catenin, upregulated the expression of c-myc and pluripotency related genes, oct4, sox2 and nanog, and blocked cell differentiation induced by all trans-RA. The proliferation of P19 cells was significantly enhanced by SB216763, as well as c-myc overexpression. C-myc downregulation inhibited P19 cell proliferation caused by activation of Wnt/β-catenin signaling and induced P19 cell differentiation. In conclusion, activation of the Wnt/β-catenin pathway could promote the proliferation and inhibit the differentiation of mouse teratocarcinoma cells by upregulation of c-myc expression.
收录类别:SCOPUS;SCIE
WOS核心被引频次:32
Scopus被引频次:36
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869507648&doi=10.1002%2far.22592&partnerID=40&md5=5e027edf6e056106097e70fd455fb094
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