标题:Label-Free Electrochemiluminescent Immunosensor for Detection of Carcinoembryonic Antigen Based on Nanocomposites of GO/MWCNTs-COOH/Au@CeO2
作者:Pang, Xuehui; Li, Jianxiu; Zhao, Yongbei; Wu, Dan; Zhang, Yong; Du, Bin; Ma, Hongmin; Wei, Qin
作者机构:[Pang, Xuehui; Li, Jianxiu; Zhao, Yongbei; Wu, Dan; Zhang, Yong; Du, Bin; Ma, Hongmin; Wei, Qin] Univ Jinan, Sch Chem & Chem Engn, Shandong Univ, Key 更多
通讯作者:Ma, HM
通讯作者地址:[Ma, HM]Univ Jinan, Sch Chem & Chem Engn, Shandong Univ, Key Lab Chem Sensing & Anal, Jinan 250022, Peoples R China.
来源:ACS APPLIED MATERIALS & INTERFACES
出版年:2015
卷:7
期:34
页码:19260-19267
DOI:10.1021/acsami.5b05185
关键词:carbon nanomaterials; modified electrodes; electrochemiluminescence;; label-free immunosensor; cancer biomarker
摘要:A high-sensitivity electrochemiluminescence (ECL) sensor was conducted to detect carcinoembryonic antigen (CEA). Nanocomposites of graphene oxide/carboxylated multiwall carbon nanotubes/gold/cerium oxide nanoparticles (GO/MWCNTs-COOH/Au@CeO2) were used as antibody carriers and sensing platforms to modify on glassy carbon electrodes (GCE). CeO2 nanopartides were first exploited as an ECL luminescent material and the possible ECL mechanism was proposed in this work. GO/MWCNTs-COOH was used as a loading matrix for CeO2 nanopartides because of the superior conductivity and large specific surface area. Au nanopartides were further deposited on this matrix to attach anti-CEA and enhance the sensitivity of immunosensor. The proposed sensing platform showed excellent cathodic ECL performance and sensitive response to CEA. The effects of experimental conditions on the ECL performance were investigated. The proposed immunosensor showed the broad linear range (0.05-100 ng/mL) and the low detection limit (LOD, 0.02 ng/mL, signal-to-noise ratio = 3) according to the selected experimental conditions. The excellent analysis performance for determination of CEA in the human serum samples simplied this immunosensor displayed high sensitivity and excellent repeatability. More importantly, this conducted immunosensor broadens the use scope of CeO2 nanopartides.
收录类别:SCIE
WOS核心被引频次:31
资源类型:期刊论文
TOP