标题:Engineered Split-TET2 Enzyme for Inducible Epigenetic Remodeling
作者:Lee, Minjung; Li, Jia; Liang, Yi; Ma, Guolin; Zhang, Jixiang; He, Lian; Liu, Yuliang; Li, Qian; Li, Minyong; Sun, Deqiang; Zhou, Y 更多
作者机构:[Lee, Minjung; Li, Jia; Zhang, Jixiang; Li, Qian; Sun, Deqiang; Huang, Yun] Texas A&M Univ, Coll Med, Ctr Epigenet & Dis Prevent, Inst Biosci & Techno 更多
通讯作者:Zhou, Yubin
通讯作者地址:[Huang, Y]Texas A&M Univ, Coll Med, Ctr Epigenet & Dis Prevent, Inst Biosci & Technol,Dept Mol & Cellular Med, 2121 W Holcombe Blvd, Houston, TX 77030 更多
来源:JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
出版年:2017
卷:139
期:13
页码:4659-4662
DOI:10.1021/jacs.7b01459
摘要:The Ten-eleven translocation (TET) family of 5-methylcytosine (5mC) dioxygenases catalyze the conversion of 5mC into 5-hydroxymethylcytosine (5hmC) and further oxidized species to promote active DNA demethylation. Here we engineered a split-TET2 enzyme to enable temporal control of 5mC oxidation and subsequent remodeling of epigenetic states in mammalian cells. We further demonstrate the use of this chemically inducible system to dissect the correlation between DNA hydroxymethylation and chromatin accessibility in the mammalian genome. This chemical-inducible epigenome remodeling tool will find broad use in interrogating cellular systems without altering the genetic code, as well as in probing the epigenotype-phenotype relations in various biological systems.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:4
Scopus被引频次:5
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016970002&doi=10.1021%2fjacs.7b01459&partnerID=40&md5=12ce86fd05ca6b6bf16d083f8b6298d7
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