标题：In vitro Biomimetic Construction of Hydroxyapatite-Porcine Acellular Dermal Matrix Composite Scaffold for MC3T3-E1 Preosteoblast Culture
作者：Zhao, Hongshi; Wang, Guancong; Hu, Shunpeng; Cui, Jingjie; Ren, Na; Liu, Duo; Liu, Hong; Cao, Chengbo; Wang, Jiyang; Wang, Zhonglin
作者机构：[Zhao, Hongshi; Wang, Guancong; Cui, Jingjie; Ren, Na; Liu, Duo; Liu, Hong; Wang, Jiyang] Shandong Univ, Sch Phys & Microelect, Ctr Bio & Micro Nano F 更多
通讯作者地址：[Liu, HB]Shandong Univ, Sch Phys & Microelect, Ctr Bio & Micro Nano Funct Mat, State Key Lab Crystal Mat, 27 Shanda Nanlu, Jinan 250100, Peoples R Chi 更多
来源：TISSUE ENGINEERING PART A
摘要：The application of porous hydroxyapatite-collagen (HAp-Collagen) as a bone tissue engineering scaffold is hindered by two main problems: its high cost and low initial strength. As a native 3-dimenssional collagen framework, purified porcine acellular dermal matrix (PADM) has been successfully used as a skin tissue engineering scaffold. Here we report its application as a matrix for the preparation of HAp to produce a bone tissue scaffold through a biomimetic chemical process. The HAp-PADMscaffold has two-level pore structure, with large channels (similar to 100 mu m in diameter) inherited from the purified PADM microstructure and small pores (< 100 nm in diameter) formed by self-assembled HAp on the channel surfaces. The obtained HAp-PADM scaffold (S15D) has a compressive elastic modulus as high as 600 kPa. The presence of HAp in sample S15D reduces the degradation rate of PADM in collagenase solution at 37 degrees C. After 7 day culture of MC3T3-E1 pre-osteroblasts, MTT data show no statistically significant difference on pure PADM framework and HAp-PADM scaffold (p>0.05). Because of its high strength and nontoxicity, its simple preparation method, and designable and tailorable properties, the HAp-PADM scaffold is expected to have great potential applications in medical treatment of bone defects.